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sp6 t7 promoter primers  (Promega)

 
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    Promega sp6 t7 promoter primers
    Sp6 T7 Promoter Primers, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sp6 t7 promoter primers/product/Promega
    Average 90 stars, based on 1 article reviews
    sp6 t7 promoter primers - by Bioz Stars, 2026-04
    90/100 stars

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    Features and use of a synthesized vector using the presented protocol for riboprobe and inhibitory RNA synthesis for planaria Smed-Slit-1 (A) The constructed vector can be used for both the synthesis of sense (with T3) and antisense (with <t>SP6)</t> riboprobes as well as dsRNA (with T7) for RNAi. (B) Sense riboprobes of Smed-Slit-1 produce no staining. (C) Antisense riboprobes of Smed-Slit-1 produce a characteristic midline expression pattern (arrows). (D) Head amputation in worms fed control dsRNA for GFP results in the regeneration of two eyes (red arrows). (E) Head amputation in worms fed dsRNA for Smed-Slit-1 results in the regeneration of a single eye at the midline (red arrow). Scale bars, 100 μm.
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    Features and use of a synthesized vector using the presented protocol for riboprobe and inhibitory RNA synthesis for planaria Smed-Slit-1 (A) The constructed vector can be used for both the synthesis of sense (with T3) and antisense (with SP6) riboprobes as well as dsRNA (with T7) for RNAi. (B) Sense riboprobes of Smed-Slit-1 produce no staining. (C) Antisense riboprobes of Smed-Slit-1 produce a characteristic midline expression pattern (arrows). (D) Head amputation in worms fed control dsRNA for GFP results in the regeneration of two eyes (red arrows). (E) Head amputation in worms fed dsRNA for Smed-Slit-1 results in the regeneration of a single eye at the midline (red arrow). Scale bars, 100 μm.

    Journal: STAR Protocols

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly

    doi: 10.1016/j.xpro.2022.101458

    Figure Lengend Snippet: Features and use of a synthesized vector using the presented protocol for riboprobe and inhibitory RNA synthesis for planaria Smed-Slit-1 (A) The constructed vector can be used for both the synthesis of sense (with T3) and antisense (with SP6) riboprobes as well as dsRNA (with T7) for RNAi. (B) Sense riboprobes of Smed-Slit-1 produce no staining. (C) Antisense riboprobes of Smed-Slit-1 produce a characteristic midline expression pattern (arrows). (D) Head amputation in worms fed control dsRNA for GFP results in the regeneration of two eyes (red arrows). (E) Head amputation in worms fed dsRNA for Smed-Slit-1 results in the regeneration of a single eye at the midline (red arrow). Scale bars, 100 μm.

    Article Snippet: SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.

    Techniques: Synthesized, Plasmid Preparation, Construct, Staining, Expressing, Control

    Journal: STAR Protocols

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly

    doi: 10.1016/j.xpro.2022.101458

    Figure Lengend Snippet:

    Article Snippet: SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.

    Techniques: Virus, Recombinant, Labeling, Purification, Plasmid Preparation, cDNA Synthesis, Software

    Journal: STAR Protocols

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly

    doi: 10.1016/j.xpro.2022.101458

    Figure Lengend Snippet:

    Article Snippet: SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.

    Techniques: Plasmid Preparation

    Riboprobe Synthesis Reaction

    Journal: STAR Protocols

    Article Title: In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly

    doi: 10.1016/j.xpro.2022.101458

    Figure Lengend Snippet: Riboprobe Synthesis Reaction

    Article Snippet: SP6 Promoter Primer , Integrated DNA Technologies, Inc , 51-01-19-05.

    Techniques: Labeling